Yes, both Cellaris and Vascular Tracker are stable at room temperature for a couple of days.

However it is strongly recommended to store all probes or solutions thereof at the recommended storage conditions of 4°C. Do not freeze our products™.

We recommend using working concentrations of 2 to 10 nM for labelling, which is in the order of multiple magnitudes lower than comparable live cell stains.

Only if above a certain threshold concentration is used, Cellaris will then start to show some effect on cell proliferation.

However, due to the inherent brightness and photostability, the probes can be used at significantly lower working concentration than ones with cytotoxic effects on cells.

Yes. With their exceptional high brightness, high cross-sectional area for two- and three- photon absorption, plus good photo-stability, giving great competitive edge to be the ideal choice for Two-Photon and Multi-photon Excitation Microscopy and they are probably the brightest probes available on the market.

All Luminicell products are delivered at ambient shipment condition.

Upon receiving the products, please store them under recommended storage conditions of 4°C.

Considering the spectral profile of Cellaris 540 (Green) and 670 (Red), DNA stains usable with a standard Cy3 fliter set are recommended.

The list below reports known working cell lines, tissues or organisms that can be stained with Cellaris, extracted from selected publications.

• Homo sapiens : U2OS, fibroblasts, HeLa, HUVEC, MCF-10A, HCT-116, A549, erythrocytes.
• Mus musculus : C2C12, IA32, skeletal muscle, primary cardiomyocyte, primary oocyte.
• Rattus norvegicus : primary hyppocampal neurons, primary cortex neurons, NRK.
• Cercopithecus aethiops : COS-7.
• Mesocricetus auratus : BHK.
• Drosophila melanogaster : Notum epithelium, S2.
• Didelphis marsupialis : OK-cells.
• Carassius auratus (goldfish) : retina bipolar cells
• Danio rerio (zebrafish) : retina bipolar cells

Please note that above list shown is non-exhaustive. Any cell line, tissue or organism is not included in list, does not translate to limitation on product staining performance.

We would greatly appreciate your valuable inputs by helping us complementing this list with any of your positive research experiences.

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No, both Cellaris and Vascular Tracker consist of lipid encapsulated organic dyes, which are already in their nanoaggregates form, kept within the core of nanoparticles.

Learn more about the dyes' functioning in our Technology section.

No, as only the fluorescence dyes within the core of the lipid nanoparticle differs between various colour options.

The surface functionalisation which are responsible for cellular uptake, cytotoxicity or cell retention does not differ between various colours.

Yes, Cellaris are able to stain cells in 3D growth environment such as spheroids and organoids.

Due to their great cellular retention and inherent high brightness and photostability, the probes are particularly suited for long term cell tracking applications.

Yes, Cellaris products are able to stain primary cells with low to minimal cytotoxicity. Validation in various experimental series can be found in Publication and Reference List.

No, Cellaris are cytosolic trackers developed for long-term live-cell staining by evenly labelling cytoplasmic matrix. The probes are efficiently taken up by cells due to their functionalized surface with cell-penetrating peptides.

Cells can be first labelled with Cellaris before fixing with PFA.

No, Cellaris are cytosolic trackers developed for long-term live-cell staining by evenly labelling cytoplasmic matrix.

The probes do not bind to sub-cellular structures, nor label sub-cellular domains such as the nucleus.

We do not use nor introduce any amount of DMSO into the products throughout the manufacturing processes.

Any other organic solvents are removed during the processes.

All Cellaris can be used with Accustase for cell detachment or cellular dissociation.

No, quite the opposite.

Our products work through an unique mechanism called Aggregation Induced Emission (AIE), where the dyes become fluorescence and enhanced in brightness once they are aggregated.

Both Cellaris and Vascular Tracker are "nano-aggregates" of AIE dyes encapsulated in lipid nanoparticles, ensuring biocompatibility and high stability.

Learn more about our Technology

Under the very high light intensities typically used in STORM imaging, phototoxicity of the excitation light is usually observed on live-cells.

SMLM techniques that require less excitation light power may be suited.