FREQUENTLY ASKED QUESTIONS
Are LuminiCell Trackers™ stable at room temperature?
Yes, LuminiCell Trackers™ are stable at room temperature for a few days. However it is strongly recommended to store all probes or solutions thereof at the recommended storage conditions of 4°C. Do not freeze LuminiCell Trackers™.
Are LuminiCell Trackers™ toxic to the cells?
We recommend using working concentrations of 2 to 10 nM for LuminiCell Trackers™, which is in the order of multiple magnitudes lower than comparable live cell stains.
Only if above a certain threshold concentration is used, LuminiCell Trackers™ will then start to show some effect on cell proliferation.
However, due to the inherent brightness and photostability, the probes can be used at significantly lower working concentration than ones with cytotoxic effects on cells.
Are LuminiCell Trackers™ compatible for multi-photon excitation imaging?
Yes. With their exceptional high brightness, high cross-sectional area for two- and three- photon absorption, plus good photo-stability, give LuminiCell Trackers™ competitive edge to be the ideal choice for Two-Photon and Multi-photon Excitation Microscopy and they are probably the brightest probes available on the market.
Learn more about applications using luminicell probes with TPE Microscopy in Publication and Reference List.
What are the shipping conditions for LuminiCell Trackers™?
All LuminiCell Trackers™ are delivered at ambient shipment condition. Upon receiving the products, please store them under recommended storage conditions of 4°C.
What dyes are recommended for complementary nuclear staining?
Considering the spectral profile of LuminiCell's green and red Trackers Cellular, DNA stains usable with a standard Cy3 fliter set are recommended.
Which organisms and tissues can be stained with LuminiCell Trackers™?
The list below reports known working cell lines, tissues or organisms that can be stained with LuminiCell Trackers™ extracted from selected publications.
- Homo sapiens : U2OS, fibroblasts, HeLa, HUVEC, MCF-10A, HCT-116, A549, erythrocytes.
- Mus musculus : C2C12, IA32, skeletal muscle, primary cardiomyocyte, primary oocyte.
- Rattus norvegicus : primary hyppocampal neurons, primary cortex neurons, NRK.
- Cercopithecus aethiops : COS-7.
- Mesocricetus auratus : BHK.
- Drosophila melanogaster : Notum epithelium, S2.
- Didelphis marsupialis : OK-cells.
- Carassius auratus (goldfish) : retina bipolar cells
- Danio rerio (zebrafish) : retina bipolar cells
Please note that above list shown is non-exhaustive. Any cell line, tissue or organism is not included in list, does not translate to limitation on product staining performance.
We would greatly appreciate your valuable inputs by helping us complementing this list with any of your positive research experiences. Drop us a message through Contact page!
Are the fluorescence characteristics of LuminiCell Trackers™ induced by aggregation of molecules inside the cells?
No, LuminiCell Trackers™ consist of lipid encapsulated organic dyes, which are already in their nanoaggregates form.
All LuminiCell Trackers™ come as lipid nanoparticles with a specified size of 30 to 100 nm and high uniformity in particle size distribution.
Learn more about the dyes' functioning in our Technology section.
Are there any differences in the biological functioning between different colour options of LuminiCell Trackers™?
No, as only the fluorescence dyes within the core of the lipid nanoparticle differs between various colour options.
The surface functionalisation which are responsible for cellular uptake, cytotoxicity or cell retention does not differ between various colours.
Can LuminiCell Trackers™ Cell Labelling Kits be used in 3D cell culture models?
Yes, LuminiCell Trackers™ Cell Labelling Kits are able to stain cells in 3D growth environment such as spheroids and organoids.
Due to their great cellular retention and inherent high brightness and photostability, the probes are particularly suited for long term cell tracking applications.
Can LuminiCell Trackers™ Cell Labelling Kits be used in primary cell cultures?
Yes, LuminiCell Trackers™ Cell Labelling Kits are able to stain primary cells with low to minimal cytotoxicity. Validation in various experimental series can be found in Publication and Reference List.
Can LuminiCell Trackers™ Cell Labelling Kits be used to label cellular membranes?
No, LuminiCell Trackers™ Cell Labelling Kits are cytosolic trackers developed for long-term live-cell staining by evenly labelling cytoplasmic matrix. The probes are efficiently taken up by cells due to their functionalized surface with cell-penetrating peptide (TAT).
Can LuminiCell Tracker™ Cell Labelling Kits be used to label fixed cells?
All LuminiCell Tracker™ Cell Labelling Kits can be used with PFA fixed cells, but do not survive well in the fixation step if the cells were labeled before the fixation.
Fixation is recommended with 4% PFA solution.
Permeabilization is recommended with 0.1% TX-100.
Can LuminiCell Trackers™ Cell Labelling Kits be used to label sub-cellular structures such as cell organelles?
No, LuminiCell Trackers™ Cell Labelling Kits are cytosolic trackers developed for long-term live-cell staining by evenly labelling cytoplasmic matrix. The probes do not bind to sub-cellular structures, nor label sub-cellular domains such as the nucleus.
Do LuminiCell Trackers™ contains any dimethyl sulfoxide (DMSO)?
We do not use nor introduce any amount of DMSO into the products throughout the manufacturing processes of LuminiCell Trackers™.
Do LuminiCell Trackers™ work with cell dissociation solutions?
All LuminiCell Tracker™ Cell Labelling Kits can be used with Accustase for cell detachment or cellular dissociation.
Do LuminiCell Trackers™ suffer from Aggregated Caused Quenching (ACQ)?
No, LuminiCell Trackers™ work through an unique mechanism called Aggregation Induced Emission (AIE), where the dyes become fluorescence and enhanced in brightness once they are aggregated.
LuminiCell Trackers™ are "nano-aggregates" of AIE dyes encapsulated in lipid nanoparticles, ensuring biocompatibility and high stability.
Learn more about LuminiCell Trackers™ functioning mechanism through our Technology page.
Is it possible to image live cells labeled with LuminiCell Trackers™ through STORM imaging?
Not possible, under the very high light intensities typically used in STORM imaging, phototoxicity of the excitation light is usually observed on live-cells. SMLM techniques that require less excitation light power may be suited.