Aggregation-Induced Emission Fluorescence Nanoparticles

LuminiCell’s proprietary technology is based on ultrabright organic materials with aggregation-induced emission (AIE) characteristics encapsulated in Lipid Nanoparticles (AIEdots) with excellent biocompatibility and strong photobleaching resistance.

Aggregation-Induced Emission (AIE)

AIE materials are highly fluorescent in their aggregated state. The fundamental behaviour of acquiring signal enhancement upon aggregation renders AIE materials distinctive from all other conventional organic molecules, where aggregation results in signal loss.

The underlying mechanism of AIE characteristics is the restriction of intramolecular motions. Conventional fluorophores, including derivates based on Cyanine-, Rhodamine-, or Fluorescein dyes lose their fluorescence in the aggregate state due to π−π stacking interaction (aggregation caused quenching or ACQ).

The dense aggregation of AIE fluorophores at the core of the lipid nanoparticles, paired with their intrinsic high extinction coefficient and quantum yield leads to the ultra-high brightness of LuminiCell dyes. LuminiCell’s dye portfolio consists of fluorophores across the visible (blue, green, red) and NIR.

Biocompatible and Uniform Encapsulation Matrix 

In our proprietary process of lipid nanoparticle synthesis, AIE fluorescence dyes is encapsulated with an amphiphilic lipid polymer matrix, with the dyes packed within the core of nano-size lipid shell structure.

The encapsulation matrix is highly biocompatible with negligible toxicity effect on cells, where the AIE dyes are now packed in highly uniform nanoparticles with a polydisperisty index of < 0.2 are formed. Our manufacturing process enables a precise control of nanoparticle size from 20-100 nm, which can be used for a variety of biological applications.

Our expertise in lipid nanoparticle encapsulation with highly optimised manufacturing process allows us to make a vast range of challenging materials biocompatible, overcoming poor colloidal stability and low water dispersibility. The lipid nanoparticle shell with various functional and targeting moieties further allows for a simple surface functionalisation for an extended range of biological use-cases.

Functionalised Surface for Targeting Ability

Through the conjugation of LuminiCell's AIEdots to antibodies and functional peptides, cellular uptake efficiency can be controlled, while selectivity for specific targeting moieties or biomarkers can be achieved. Different targeting groups and functional moieties can be made readily available on the surface of AIEdots, providing great convenience and enabling ease of conjugation to antibodies or peptides of interest.

We are well-experienced in functionalization of our AIEdots, spanning across common conjugation methods such as Thiol-reactive maleimide dyes, amine-reactive NHS-ester, as well as Clickable alkyne and Clickable azide groups.

Product Validation

LuminiCell Tracker are formulated with our proprietary AIEdots technology, and have been applied across different applications, for instances: long-term live cell tracking, deep tissue bioimaging, which includes intravital vascular tracking and diagnostic immunoassays.

See References for a list of publications with LuminiCell dyes and latest application notes.

Get to Know More

At Lumincell, we are dedicated to provide our customers with the best performing fluorescent nanoparticles, unlocking information that are now made achievable with our products, yielding superior results to accelerate all fields of biomedical research.

Our platform technology are highly tuneable, allowing users to achieve their application needs. 

Get in touch with us to tackle your individual application with custom products of LuminiCell’s AIEdots.