Luminicell Tracker™ 670

Cellular Labelling Kit (Red)

Luminicell Tracker™ 670 - Cell Labelling Kit (Red)

Description

Luminicell Tracker™ are highly emissive fluorescent organic nanoparticles, built for rapid labelling of live cells with great biocompatibility. They can be readily taken up by various types of cells within 1 hour. Once taken up by cells, they show intense luminescence with high photo-stability and possess durable signal for many cell generations with negligible cell toxicity.

Our cell labelling kit offers 3X longer tracking duration and 10X brighter in fluorescence signal as compared to competitive products. The labelled cells can be monitored using a variety of fluorescence techniques such as confocal microscopy, multi-photon microscopy and flow cytometry.

Luminicell Tracker™ 670 - Cell Labelling Kit contain red fluorescent AIEDot nanoparticles with TAT peptide sequences. These nanoparticles can be used to tag cells for long term fluorescence cell experiments.

Each labelling kit allows up to 100 staining experiments.

Key Features

Rapid labelling of < 1 hr with high cell labelling rate of > 99%
High brightness with 10X brighter signal than other cell labelling kits
High biocompatibility of > 95% cell viability after 48 hours
High photostability and signal retention of > 90 % after 10 mins
Good cellular retention, zero signal cross-talk
Durable tracking ability of > 10 cell generations (in-vitro) and 21 days (in-vivo) for cancer cells

Applications

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments.

Upon labeling, the long-lasting nature of Luminicell Tracker™ allows a wide variety of cell tracking applications both in vitro and in small animal models.

Cell differentiation, cell mobility, cell-cell interaction, etc. can be well studied with Luminicell Tracker™. These nanoparticles have excellent cell retention and are not known to disrupt normal cell functions.

Luminicell Tracker™ labeled cells can be monitored using a variety of fluorescence techniques such as confocal microscopy and flow cytometry.

Specifications

500 ul of 200 nM AIEdots (size: 30 ± 5 nm)

Compatible with laser line of 458/488/543 nm

Working concentration of 2 nM for labelling

Optical Properties

λ_abs = 506 nm

λ_fl = 670 nm

ε_max = 1.7 x 10⁷ M^-1·cm^-1

Quantum Yield ≥ 18%

Tested with cellular assay: HeLa Cell Fluorescence

Storage and Stability

Store at -2-8°C upon receipt. Do not freeze product.

Thaw at room temperature or in a water bath before use.

Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing Luminicell Tracker™ three times for 1 min each before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

References

Liu B, Tang BZ et al. Photostable fluorescent organic dots with aggregation-induced emission (AIE dots) for noninvasive long-term cell tracing. Sci Rep. 2013;3:1150.

Kang Y et al. Long-Term Tracking Mesenchymal Stem Cell Differentiation with Photostable Fluorescent Nanoparticles. ACS Appl Mater Interfaces. 2016 May 18;8(19):11925-33.